RBS

Part:BBa_K4286527:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-09-28)


cbh1 leader sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Pcdh1-cdh1ls-mazEF-Tcdh1 circuit was integrated into pCAMBIA1302 vector by homologous recombination. We transformed the recombinant plasmid into Trichoderma atroviride by Agrobacterium-mediated transformation in order to achieve condition-triggered suicide of T. atroviride ultimately.


Source

The sequence of cbh1 leader sequence belongs to Trichoderma koningii cbh1 gene for 1,4-beta-D-glucan-cellobiohydrolase (GenBank: X69976.1), which comes from genomic sequence of Trichoderma koningii.


References

[1]Wey TT, Hseu TH, Huang L. Molecular cloning and sequence analysis of the cellobiohydrolase I gene from Trichoderma koningii G-39. Curr Microbiol. 1994 Jan;28(1):31-9. doi: 10.1007/BF01575983.

[2]Karhunen T, Mäntylä A, Nevalainen KM, Suominen PL. High frequency one-step gene replacement in Trichoderma reesei. I. Endoglucanase I overproduction. Mol Gen Genet. 1993 Dec;241(5-6):515-22. doi: 10.1007/BF00279893.